RBS
T7 0.3

Part:BBa_B2001:Design

Designed by: Heather Keller   Group: T7.2   (2006-09-27)


T7 RBS 0.3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The -20 to -1 region was chosen to be representative of the RBS region of the T7 genes for the following reasons: 1) The Shine-Delgarno region is contained within this defined region for all T7 genes. 2) Current literature suggests that when bound to the Translation Start Site (0), the Ribosome covers positions -20 to +13 of the gene.

Source

Genome of wild-type bacteriophage T7, positions 905-924. Includes the -20 to -1 region of gp0.3.

References

  1. Dunn, J.J. and Studier F.W. (1983) JMB 166, 477-535.
  2. Ringquist, S., Shinedling, S., Barrick, D., Green, L., Binklet, J., Stormo, G.D., and Gold, L. (1992) Mol. Microbio. 6, 1219-1229.
  3. T7 Genome, [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi??db=nucleotide&val=V01146 Genbank #V01146]